ABCB4 exports phosphatidylcholine in a sphingomyelin-dependent manner
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چکیده
منابع مشابه
Densitometry of phosphatidylcholine and sphingomyelin in high-density lipoproteins.
We describe the quantitative densitometric determination of phosphatidylcholine (PC) and sphingomyelin (SP) in human serum after precipitation with phosphotungstic acid/MgCl2 and use of thin-layer chromatography. After development, chromatographic plates were charred with methanolic sulfuric acid and MnCl2 and scanned by direct reflectance densitometry in an automated densitometric system inter...
متن کاملImaging of the domain organization in sphingomyelin and phosphatidylcholine monolayers.
The lateral organization of biomembranes has gained significant interest when the fluid mosaic model was challenged by the model of "lipid rafts". Several lipid classes like cholesterol and sphingolipids are considered to be essential for their formation. Here we investigate the lateral domain formation in binary mixtures of sphingomyelin and phosphatidylcholine. Both are major lipid components...
متن کاملRapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine.
Sphingomyelin (SM) and phosphatidylcholine (PC) are two major phospholipids on plasma lipoproteins. Their concentration is classically measured by lipid extraction, thin-layer chromatography, and phosphate determination on separated SM or PC spots. Here, we describe two rapid, specific, and sensitive enzymatic measurements for both phospholipids. Plasma was incubated with bacterial sphingomyeli...
متن کاملSphingomyelin/phosphatidylcholine and cholesterol interactions studied by imaging mass spectrometry.
Label-free imaging mass spectrometry is utilized the first time to study lipid-lipid interactions in a model membrane system. Ternary lipid mixtures of cholesterol (CH), sphingomyelin (SM), and phosphatidylcholine (PC) on supported Langmuir-Blodgett films are investigated as a mimic of the cellular membrane. The unique chemical specificity and imaging capability allow identification and localiz...
متن کاملEgg-sphingomyelin and cholesterol form a stoichiometric molecular complex in bilayers of egg-phosphatidylcholine.
Sphingomyelin and cholesterol are membrane lipids that interact to form liquid-ordered phase believed to act as a platform for the organization of signaling proteins. We report analyses of synchrotron X-ray powder diffraction patterns recorded from aqueous dispersions of ternary mixtures of sphingomyelin and phosphatidylcholine from egg yolk and cholesterol to investigate how cholesterol distri...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 2015
ISSN: 0022-2275
DOI: 10.1194/jlr.m056622